How to: Submit sequence data to NCBI
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SUBMISSION TOOLS |
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Simple Sequence Submissions |
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Single nucleotide sequence or Several nucleotide sequences for different genes or loci |
Contiguous bases of cDNA or genomic DNA, but should not be complete genomes. Complete genomes should be submitted via the appropriate protocol indicated below. Records with simple annotation may be submitted by BankIt or Sequin, while records with complicated annotation may be more easily submitted via Sequin. |
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Group of nucleotide sequences for the same gene or locus |
Includes:
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Batches of Sequences |
Includes:
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Genomic Assembly Submissions |
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Small complete genomes |
Includes chloroplasts, mitochondria, plasmids, phages, and viruses |
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Large complete genomes |
Includes paired chromosome and plasmids, as well as bacterial or eukaryotic chromosomes Questions regarding a specific submission that are not answered in the documented instructions can be sent to genomes@ncbi.nlm.nih.gov . |
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Incomplete genomes |
These can be whole genome shotgun (WGS) sequences. WGS submissions should be prepared using the tbl2asn or Sequin tools. For assistance contact genomes@ncbi.nlm.nih.gov . |
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High Throughput Genome Sequences (HTGSs) |
The clones (e.g. BACs) of large-scale clone-based genome sequencing projects that are to be released quickly into GenBank can be submitted via the HTGS system. Sequences that are to be kept confidential or are few in number should be submitted as described above for Single nucleotide sequences. HTGS submissions require prior communication with NCBI staff, so please read about the HTGS submission process for details. |
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Other Submission Types |
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Barcode of Life sequences |
Mitochondrial cytochrome oxidase I sequences that are part of the Barcode of Life initiative can be submitted using a customized Bankit. |
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New sequence annotation for a non-RefSeq record submitted to GenBank by someone else |
Third Party Annotation (TPA) submissions can be created for annotation of existing GenBank records when the submitter has experimental or inferential evidence that will be published in a peer-reviewed biological journal. Please read about the TPA database and its submissions policies before submission. |
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Computationally assembled transcript sequences |
These records, based on those that have already been submitted to SRA or the Trace Archive, may be candidates for submission to the Transcriptome Shotgun Assembly (TSA) repository. |
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Variations or Polymorphisms1 |
Single nucleotide polymorphisms as well as short insertions and deletions (<50bp) should be submitted to dbSNP, while large structural variations and copy number variation (CNV) data should be submitted to dbVar. Please note that human variations/polymorphisms with clinical relevance should be submitted to a specialized Human Variation Batch submission process using HGVS nomenclature. |
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Primers, siRNAs, or probes |
Primer or nucleotide-based probe sequences should be submitted to the Probe Database. |
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High throughput sequences |
The Sequence Read Archive (SRA) accepts reads from high throughput sequencing instruments. Some submissions include sets of SRA reads as part of a comprehensive package. For the specific datasets described below, please initiate submissions with the appropriate archive:
Curators of these resources will assist submitters in sending the data to SRA during the submission process. |
For data types not mentioned to the left, submit directly to SRA: |
1If you need a GenBank accession number for Variation or Polymorphism submissions, you will need to annotate the variations as SNPs, insertions/deletions, or microsatellite regions on a nucleotide sequence and submit this to GenBank using the appropriate mechanism for the sequence type.